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Serum level of micronutrients mg, Se, zn, cu and antioxidant enzymes Gpx1and Cu/Zn SOD, in Sudanese asthmatics and their relationship with the severity of asthma

by Magboula Mamoun Hussein Sherief; Khartoum University Faculty of medical and Health Studies Board -- Dept. Human Physiology.
Type: materialTypeLabelBookPublisher: 2011Description: xiii,94p. : ill, : 30cm.Subject(s): Human physiology | Asthma -- microonutrients | Asthma -- epidemiology | Asthma -- prevalence | Asthma -- antioxidant enzymesOnline resources: Click here to access online Dissertation note: M. D. 2011 Summary: Background: Asthma is a bronchial inflammatory disease. Asthmatic patients tend to have increased oxidative stress caused by imbalance between oxidants and antioxidants. Micronutrients are essential components of antioxidant enzymes that eliminate excess overproduced oxidants. Objectives: To determine serum level of micronutrients Se, Zn, Cu, Mg, and antioxidant enzymes Gpx1 and Cu/Zn SOD in asthma patients and to compare them with the level in healthy controls. Further, to elucidate if the severity of asthma and the acute attack would change the level of these micronutrients and antioxidant enzymes. Methods: This is a case control hospital based study conducted during the period of 2006-2009 among 196 cases 97 females and 99 males, and 225 controls, 90 females and 135 males. The age range of the study subjects was between 18-70 years old with a mean of 37.5 ± 13.5 (mean ± SD). Patients were selected from asthma clinics and emergency asthma rooms in AL Shaab and Omdurman Teaching hospitals. Information about demographic and clinical characteristics was collected from each patient using a questionnaire. The work was conducted in two stages. In stage 1, serum Mg, Se, Zn, and Cu were measured in asthmatic patients, most of them were selected from emergency rooms while introduced to asthma therapies, and from controls. In stage 2, serum micronutrients Se, Zn, Cu and their related antioxidant enzymes were evaluated in groups of patients who were clinically classified according to asthma severity to severe and mild cases, and according to the existence of steady state and acute exacerbation into patients during acute attack and patients in a stable period. Serum micronutrients were measured using atomic absorption spectrophotometric methods. Serum antioxidant enzymes Gpx1 and Cu/Zn SOD were measured using ELIZA kits. Results: In stage 1, mean serum Zn was significantly higher in patients than in controls (1.2 mg/L and 0.94 mg/L) respectively. Mean serum Se was significantly lower in patients compared to controls (0.65 mg/L and 0.78 mg/L) respectively. Mean serum Cu was significantly lower in patients than in controls(0.62 mg/L and 0.82 mg/L) respectively, while there was no significant difference in serum Mg compared to controls (1.644 mg/L and 1.634 mg/L) respectively. In stage 2, when clinically grouping the patients, serum Se, Zn, and Cu were found significantly low (p= 0.001) in severe asthmatic patients compared to controls. Serum Se and Zn were increased, but not statistically significant, during acute exacerbation and introduction of asthma therapies, while serum Cu did not change. Serum antioxidant enzyme Cu/Zn SOD was low in patients compared to controls, with a lower level during acute attack , while increased levels in stable and severe cases were found but the differences were not statistically significant. Serum antioxidant enzyme Gpx1 was found significantly low in patients compared to controls, and significantly low of cases in acute attack compared to stable cases (p= 0.001), while it was low in severe cases but not statistically significant. Conclusion and recommendations: These results showed that, there was deficiency in the antioxidant enzymes Cu/ZnSOD and Gpx1 and their related micronutrients Zn and Se, in asthmatic patients compared to controls. The results also showed improvement in Cu/ZnSOD in the severe cases that were subjected to regular therapies. Zn increase in cases during the acute phase, while they were introduced to asthma drugs. This suggests that proper supplementations of these micronutrients might increase the efficiency of the antioxidant defense system. Regular treatment with asthma drugs might decrease the oxidant stress in these patients by increasing the accumulations of the micronutrients and their related antioxidant enzymes. Q95Summary: هنالك دور كامن للمؤكسدات في نشاة او تولد مرض الربو الشعبي خاصة اثناء اثارتة او تفاقمه. وان المعلومات البحثية الموجودة قد اثبتت ان العناصر الضيلة و مضادات الاكسدة المرتبطه بها قد يكون لها دور في علاج مرض الربو الشعبي وخصوصا عند تفاقمه. الاهداف: هذه الدراسة صممت لتحديد قيم مستوي العناصر الضئيله وهي ,الزنك ,النحاس, السلينوم و المغينزيوم و الانزيمات مضادات الاكسده وهي Gpx و Cu Zn SOD في سيرم مرض الربو الرئوي ومقارنته بسيرم من اشخاص اصحاء. وتوضيح التغير الذي يطرا لمستوئ هذه المواد من السيرم فى مختلف درجات تفاقم المرض. الاساليب: هذة دراسه حالة ضابط اساسها المستشفئ .اجريت في مستشفي الشعب و امدرمان التعليمي من الفتره بين 2009- 2006. وقد تضمنت 196 حالة و 225 ضابط تتراوح اعمارهم بين 18-70 سنة متوسط 13.05 + 37.5 .ثم اختيار الحالات من العيادات الخاصه وغرف الطؤاري العيادات المحولة. المعلومات الاحصائيه والطبية التي تتعلق بتاريخ المرض والادويه قد تم جمعها بواسطة استبيان و بمساعدة ملفات المرض بغرض تصنيف المرض. الحالات تتضمن مرضى مصنفين حسب التقارير بملفاتهم ( مرضي حاد وضعيف) وايضا هنالك مرضئ غير مصنيفين ولكن غالبيتهم كانوا مرض موجودين بغرف الطؤاري لتلغي العلاج وهم في حالة تفاقم او اثاره للمرض. مقياس مستوي العناصر الضئيله (زنك - سلينوم - نحاس – مغينزيم ) اجري بواسطة استخدام جهاز الامتصاص الذري. ومقياسات مستوي الانزيمات مضادات الاكسده اجريت بواسطة استخدام مجموعات الاليزا. النتائج : مجموعه الحالات مقارنة بالضوابط كشفت عن انخفاض ملحوظ في قيم السيلنوم والزنك مع تحسين المستوي اثناء تفاقم المرض وتعاطي العلاج .وايضا النحاس فهو منحفض انخفاض ملحوظ من كل الحالات ولكن لم يتغير اثناء تفاقم المرض. بالنسبه للمغينزم ليس هنالك اختلاف بين الحالات والضوابط. انزيم ال CU/Zn SOD كشف عند انخفاض في مجموعة الحالات مقارنة بالضوابط مع اكثر انخفاضا ولكن يرتفع في الحالات الحاده والمستقره. انزيم ال GPX1 كشف عن انخفاض ملحوظ من جميع حالات المرض مقارنة بالضوابط. الاستنتاج والتوضيح: هذه النتائج اوضحت وجود وطاة اكسده تصاحب مرض الربو الشعبي .وان سعة الدفاع المضاده لمفعول المؤكسدات منخفضه وهذا بوازي الانخفاض فى مستوي السلنينوم والزنك - البرامج الغذائية موصي بها بالاهتمام بتجنب انخفاض هذ ه العناصر بالدم وتعويضها حتي تزداد سعة الجسم فى الدفاع عن المؤكسداد. Q95
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Item type Location Call number Status Date due Barcode
Thesis & Dissertation Thesis & Dissertation
مكتبة مجلس الدراسات الطبية و الصحية
612.2 MAG (Browse shelf) Available mh1515

A thesis submitted the degree of ph D. in Human Physiology

M. D. 2011

Includes bibliographical references

Background: Asthma is a bronchial inflammatory disease. Asthmatic patients tend to have increased oxidative stress caused by imbalance between oxidants and antioxidants. Micronutrients are essential components of antioxidant enzymes that eliminate excess overproduced oxidants. Objectives: To determine serum level of micronutrients Se, Zn, Cu, Mg, and antioxidant enzymes Gpx1 and Cu/Zn SOD in asthma patients and to compare them with the level in healthy controls. Further, to elucidate if the severity of asthma and the acute attack would change the level of these micronutrients and antioxidant enzymes. Methods: This is a case control hospital based study conducted during the period of 2006-2009 among 196 cases 97 females and 99 males, and 225 controls, 90 females and 135 males. The age range of the study subjects was between 18-70 years old with a mean of 37.5 ± 13.5 (mean ± SD). Patients were selected from asthma clinics and emergency asthma rooms in AL Shaab and Omdurman Teaching hospitals. Information about demographic and clinical characteristics was collected from each patient using a questionnaire. The work was conducted in two stages. In stage 1, serum Mg, Se, Zn, and Cu were measured in asthmatic patients, most of them were selected from emergency rooms while introduced to asthma therapies, and from controls. In stage 2, serum micronutrients Se, Zn, Cu and their related antioxidant enzymes were evaluated in groups of patients who were clinically classified according to asthma severity to severe and mild cases, and according to the existence of steady state and acute exacerbation into patients during acute attack and patients in a stable period. Serum micronutrients were measured using atomic absorption spectrophotometric methods. Serum antioxidant enzymes Gpx1 and Cu/Zn SOD were measured using ELIZA kits. Results: In stage 1, mean serum Zn was significantly higher in patients than in controls (1.2 mg/L and 0.94 mg/L) respectively. Mean serum Se was significantly lower in patients compared to controls (0.65 mg/L and 0.78 mg/L) respectively. Mean serum Cu was significantly lower in patients than in controls(0.62 mg/L and 0.82 mg/L) respectively, while there was no significant difference in serum Mg compared to controls (1.644 mg/L and 1.634 mg/L) respectively. In stage 2, when clinically grouping the patients, serum Se, Zn, and Cu were found significantly low (p= 0.001) in severe asthmatic patients compared to controls. Serum Se and Zn were increased, but not statistically significant, during acute exacerbation and introduction of asthma therapies, while serum Cu did not change. Serum antioxidant enzyme Cu/Zn SOD was low in patients compared to controls, with a lower level during acute attack , while increased levels in stable and severe cases were found but the differences were not statistically significant. Serum antioxidant enzyme Gpx1 was found significantly low in patients compared to controls, and significantly low of cases in acute attack compared to stable cases (p= 0.001), while it was low in severe cases but not statistically significant. Conclusion and recommendations: These results showed that, there was deficiency in the antioxidant enzymes Cu/ZnSOD and Gpx1 and their related micronutrients Zn and Se, in asthmatic patients compared to controls. The results also showed improvement in Cu/ZnSOD in the severe cases that were subjected to regular therapies. Zn increase in cases during the acute phase, while they were introduced to asthma drugs. This suggests that proper supplementations of these micronutrients might increase the efficiency of the antioxidant defense system. Regular treatment with asthma drugs might decrease the oxidant stress in these patients by increasing the accumulations of the micronutrients and their related antioxidant enzymes. Q95

هنالك دور كامن للمؤكسدات في نشاة او تولد مرض الربو الشعبي خاصة اثناء اثارتة او تفاقمه. وان المعلومات البحثية الموجودة قد اثبتت ان العناصر الضيلة و مضادات الاكسدة المرتبطه بها قد يكون لها دور في علاج مرض الربو الشعبي وخصوصا عند تفاقمه. الاهداف: هذه الدراسة صممت لتحديد قيم مستوي العناصر الضئيله وهي ,الزنك ,النحاس, السلينوم و المغينزيوم و الانزيمات مضادات الاكسده وهي Gpx و Cu Zn SOD في سيرم مرض الربو الرئوي ومقارنته بسيرم من اشخاص اصحاء. وتوضيح التغير الذي يطرا لمستوئ هذه المواد من السيرم فى مختلف درجات تفاقم المرض. الاساليب: هذة دراسه حالة ضابط اساسها المستشفئ .اجريت في مستشفي الشعب و امدرمان التعليمي من الفتره بين 2009- 2006. وقد تضمنت 196 حالة و 225 ضابط تتراوح اعمارهم بين 18-70 سنة متوسط 13.05 + 37.5 .ثم اختيار الحالات من العيادات الخاصه وغرف الطؤاري العيادات المحولة. المعلومات الاحصائيه والطبية التي تتعلق بتاريخ المرض والادويه قد تم جمعها بواسطة استبيان و بمساعدة ملفات المرض بغرض تصنيف المرض. الحالات تتضمن مرضى مصنفين حسب التقارير بملفاتهم ( مرضي حاد وضعيف) وايضا هنالك مرضئ غير مصنيفين ولكن غالبيتهم كانوا مرض موجودين بغرف الطؤاري لتلغي العلاج وهم في حالة تفاقم او اثاره للمرض. مقياس مستوي العناصر الضئيله (زنك - سلينوم - نحاس – مغينزيم ) اجري بواسطة استخدام جهاز الامتصاص الذري. ومقياسات مستوي الانزيمات مضادات الاكسده اجريت بواسطة استخدام مجموعات الاليزا. النتائج : مجموعه الحالات مقارنة بالضوابط كشفت عن انخفاض ملحوظ في قيم السيلنوم والزنك مع تحسين المستوي اثناء تفاقم المرض وتعاطي العلاج .وايضا النحاس فهو منحفض انخفاض ملحوظ من كل الحالات ولكن لم يتغير اثناء تفاقم المرض. بالنسبه للمغينزم ليس هنالك اختلاف بين الحالات والضوابط. انزيم ال CU/Zn SOD كشف عند انخفاض في مجموعة الحالات مقارنة بالضوابط مع اكثر انخفاضا ولكن يرتفع في الحالات الحاده والمستقره. انزيم ال GPX1 كشف عن انخفاض ملحوظ من جميع حالات المرض مقارنة بالضوابط. الاستنتاج والتوضيح: هذه النتائج اوضحت وجود وطاة اكسده تصاحب مرض الربو الشعبي .وان سعة الدفاع المضاده لمفعول المؤكسدات منخفضه وهذا بوازي الانخفاض فى مستوي السلنينوم والزنك - البرامج الغذائية موصي بها بالاهتمام بتجنب انخفاض هذ ه العناصر بالدم وتعويضها حتي تزداد سعة الجسم فى الدفاع عن المؤكسداد. Q95

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